› 2e3o

X-ray diffraction
1.55Å resolution

Crystal structure of CERT START domain in complex with C16-ceramide (P212121)

Released:
DOI: 10.2210/pdb2e3o/pdb
PDB entry 2e3o coloured by chain, front view.
PDB entry 2e3o coloured by chain, side view.
PDB entry 2e3o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R
Proc Natl Acad Sci U S A 105 488-93 (2008)
PMID: 18184806

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide transfer protein Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 347-624; Coverage: 40%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: START domain
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand 16C 1 x 16C
Ligand DMS 2 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 55.958Å b: 57.068Å c: 88.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.218
Expression system: Escherichia coli

Quick links

Citations

48 review citations

An overview of sphingolipid metabolism: from synthesis to breakdown.
Gault et al. (2010)
47 more

8 mentions without citation

Mechanisms of nonvesicular lipid transport.
Reinisch et al. (2021)
7 more