› 2e3p

X-ray diffraction
1.4Å resolution

Crystal structure of CERT START domain in complex with C16-cearmide (P1)

Released:
DOI: 10.2210/pdb2e3p/pdb
PDB entry 2e3p coloured by chain, front view.
PDB entry 2e3p coloured by chain, side view.
PDB entry 2e3p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R
Proc Natl Acad Sci U S A 105 488-93 (2008)
PMID: 18184806

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide transfer protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 347-624; Coverage: 40%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: START domain
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand 16C 2 x 16C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 45.976Å b: 49.699Å c: 62.365Å
α: 77.72° β: 71.06° γ: 82.1°
R-values:
R R work R free
0.219 0.217 0.251
Expression system: Escherichia coli

Quick links

Citations

49 review citations

An overview of sphingolipid metabolism: from synthesis to breakdown.
Gault et al. (2010)
48 more

5 mentions without citation

Inter-organelle ER-endolysosomal contact sites in metabolism and disease across evolution.
Hariri et al. (2016)
4 more