› 2e7j

X-ray diffraction
2.4Å resolution

Crystal Structure of Sep-tRNA:Cys-tRNA Synthase from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2e7j/pdb
PDB entry 2e7j coloured by chain, front view.
PDB entry 2e7j coloured by chain, side view.
PDB entry 2e7j coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus.
Fukunaga R, Yokoyama S
J Mol Biol 370 128-41 (2007)
PMID: 17512006

Function and Biology Details

Reaction catalysed: 
O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 41.99 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O30207 (Residues: 1-371; Coverage: 100%)
Gene name: AF_0028
Sequence domains: O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P41212
Unit cell:
a: 152.174Å b: 152.174Å c: 92.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA.
Kato et al. (2017)
6 more

3 mentions without citation

RNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea.
Mukai et al. (2017)
2 more