› 2e87

X-ray diffraction
2.35Å resolution

Crystal structure of hypothetical GTP-binding protein PH1320 from Pyrococcus horikoshii OT3, in complex with GDP

Released:
DOI: 10.2210/pdb2e87/pdb
PDB entry 2e87 coloured by chain, front view.
PDB entry 2e87 coloured by chain, side view.
PDB entry 2e87 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Kukimoto-Niino M, Bessho Y, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
OBG-type G domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 41.7 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59046 (Residues: 1-357; Coverage: 100%)
Gene name: PH1320
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GDP 1 x GDP
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 47.253Å b: 99.191Å c: 126.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.255
Expression system: Escherichia coli

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