› 2e89

X-ray diffraction
2.5Å resolution

Crystal structure of Aquifex aeolicus TilS in a complex with ATP, Magnesium ion, and L-lysine

Released:
DOI: 10.2210/pdb2e89/pdb
PDB entry 2e89 coloured by chain, front view.
PDB entry 2e89 coloured by chain, side view.
PDB entry 2e89 coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Kuratani M, Yoshikawa Y, Takahashi S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(Ile)-lysidine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 37.39 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67728 (Residues: 1-317; Coverage: 100%)
Gene names: aq_1887, tilS
Sequence domains: PP-loop family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand LYS 1 x LYS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 97.42Å b: 81.57Å c: 109.11Å
α: 90° β: 105.98° γ: 90°
R-values:
R R work R free
0.229 0.229 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Discovery and characterization of tRNAIle lysidine synthetase (TilS).
Suzuki et al. (2010)
5 more

3 mentions without citation

Convergent evolution of AUA decoding in bacteria and archaea.
Suzuki et al. (2014)
2 more