› 2e9f

X-ray diffraction
2.8Å resolution

Crystal Structure of T.th.HB8 Argininosuccinate lyase complexed with L-Arginine

Released:
DOI: 10.2210/pdb2e9f/pdb
PDB entry 2e9f coloured by chain, front view.
PDB entry 2e9f coloured by chain, side view.
PDB entry 2e9f coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry author: Goto M

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 462 amino acids
Theoretical weight: 51.99 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLL0 (Residues: 1-462; Coverage: 100%)
Gene names: TTHA0283, argH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ARG 4 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 78.336Å b: 119.787Å c: 257.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.271
Expression system: Escherichia coli BL21(DE3)

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