› 2eay

X-ray diffraction
1.95Å resolution

Crystal Structure Of Biotin Protein Ligase From Aquifex Aeolicus

Released:
DOI: 10.2210/pdb2eay/pdb
PDB entry 2eay coloured by chain, front view.
PDB entry 2eay coloured by chain, side view.
PDB entry 2eay coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Bagautdinov B, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
biotin--[biotin carboxyl-carrier protein] ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.79 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66837 (Residues: 1-233; Coverage: 100%)
Gene names: aq_566, birA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 55.076Å b: 60.589Å c: 73.106Å
α: 90° β: 91.99° γ: 90°
R-values:
R R work R free
0.214 0.214 0.247
Expression system: Escherichia coli

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