› 2eb0

X-ray diffraction
2.2Å resolution

Crystal structure of Methanococcus jannaschii putative family II inorganic pyrophosphatase

Released:
DOI: 10.2210/pdb2eb0/pdb
PDB entry 2eb0 coloured by chain, front view.
PDB entry 2eb0 coloured by chain, side view.
PDB entry 2eb0 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Entry authors: Shimada A, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese-dependent inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.16 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58025 (Residues: 1-307; Coverage: 100%)
Gene names: MJ0608, ppaC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 72.843Å b: 146.761Å c: 54.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.271
Expression system: Escherichia coli

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