› 2eb1

X-ray diffraction
2Å resolution

Crystal Structure of the C-Terminal RNase III Domain of Human Dicer

Released:
DOI: 10.2210/pdb2eb1/pdb
PDB entry 2eb1 coloured by chain, front view.
PDB entry 2eb1 coloured by chain, side view.
PDB entry 2eb1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer.
Takeshita D, Zenno S, Lee WC, Nagata K, Saigo K, Tanokura M
J Mol Biol 374 106-20 (2007)
PMID: 17920623

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease Dicer Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPY3 (Residues: 1659-1852; Coverage: 10%)
Gene names: DICER, DICER1, HERNA, KIAA0928
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease III domain

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 88.602Å b: 199.743Å c: 119.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.234
Expression system: Escherichia coli

Quick links

Citations

9 review citations

From guide to target: molecular insights into eukaryotic RNA-interference machinery.
Ipsaro et al. (2015)
8 more

11 mentions without citation

A Structural View of miRNA Biogenesis and Function.
Leitão et al. (2022)
10 more