› 2eeo

X-ray diffraction
1.6Å resolution

Crystal Structure of T.th. HB8 L-Aspartate-alpha-Decarboxylase Complexed with Fumarate

Released:
DOI: 10.2210/pdb2eeo/pdb
PDB entry 2eeo coloured by chain, front view.
PDB entry 2eeo coloured by chain, side view.
PDB entry 2eeo coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry author: Goto M

Function and Biology Details

Reaction catalysed: 
L-aspartate = beta-alanine + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178100 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate 1-decarboxylase beta chain Chain: A
Molecule details ›
Chain: A
Length: 24 amino acids
Theoretical weight: 2.82 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKN7 (Residues: 1-24; Coverage: 20%)
Gene names: TTHA0606, panD
Aspartate 1-decarboxylase alpha chain Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.23 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKN7 (Residues: 26-120; Coverage: 79%)
Gene names: TTHA0606, panD
Sequence domains: Aspartate decarboxylase
Structure domains: Barwin-like endoglucanases

Ligands and Environments

2 bound ligands:
Ligand PYR 1 x PYR
Ligand FUM 1 x FUM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I4
Unit cell:
a: 68.118Å b: 68.118Å c: 48.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.2
Expression system: Escherichia coli BL21(DE3)

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