› 2eez

X-ray diffraction
2.71Å resolution

Crystal structure of alanine dehydrogenase from themus thermophilus

Released:
DOI: 10.2210/pdb2eez/pdb
PDB entry 2eez coloured by chain, front view.
PDB entry 2eez coloured by chain, side view.
PDB entry 2eez coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kumarevel TS, Karthe P, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine dehydrogenase Chains: A, C, D, E, F, G
Molecule details ›
Chains: A, C, D, E, F, G
Length: 369 amino acids
Theoretical weight: 38.68 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLS7 (Residues: 1-369; Coverage: 100%)
Gene name: TTHA0216
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 133.94Å b: 133.24Å c: 147.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.264
Expression system: Escherichia coli BL21(DE3)

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