› 2efq

X-ray diffraction
2.3Å resolution

Crystal Structure of Thr134 to Ala of ST1022-Glutamine Complex from Sulfolobus tokodaii 7

Released:
DOI: 10.2210/pdb2efq/pdb
PDB entry 2efq coloured by chain, front view.
PDB entry 2efq coloured by chain, side view.
PDB entry 2efq coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding.
Kumarevel T, Nakano N, Ponnuraj K, Gopinath SC, Sakamoto K, Shinkai A, Kumar PK, Yokoyama S
Nucleic Acids Res 36 4808-20 (2008)
PMID: 18653535

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH asnC-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.47 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F9VNT4 (Residues: 1-150; Coverage: 100%)
Gene names: ST1022, STK_10220, grp
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GLN 1 x GLN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: I422
Unit cell:
a: 103.946Å b: 103.946Å c: 75.039Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Role of the Extremolytes Ectoine and Hydroxyectoine as Stress Protectants and Nutrients: Genetics, Phylogenomics, Biochemistry, and Structural Analysis.
Czech et al. (2018)
4 more

1 mention without citation

Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding.
Kumarevel et al. (2008)