2efr

X-ray diffraction
1.8Å resolution

Crystal structure of the c-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 1.8 angstroms resolution

Released:

Function and Biology Details

Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-136513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Tropomyosin alpha-1 chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 18.34 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03069 (Residues: 249-277; Coverage: 10%)
  • Canonical: P58772 (Residues: 176-273; Coverage: 35%)
Gene names: AAS101, AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P1
Unit cell:
a: 42.363Å b: 65.84Å c: 71.135Å
α: 66.4° β: 89.93° γ: 90.03°
R-values:
R R work R free
0.237 0.237 0.316
Expression system: Escherichia coli BL21(DE3)