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X-ray diffraction
2Å resolution

Crystal structure of the C-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 2.0 angstroms resolution

Released:
DOI: 10.2210/pdb2efs/pdb
PDB entry 2efs coloured by chain, front view.
PDB entry 2efs coloured by chain, side view.
PDB entry 2efs coloured by chain, top view.
Source organisms:
Entry authors: Minakata S, Nitanai Y, Maeda K, Oda N, Wakabayashi K, Maeda Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Tropomyosin alpha-1 chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 18.34 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03069 (Residues: 249-277; Coverage: 10%)
  • Canonical: P58772 (Residues: 176-273; Coverage: 35%)
Gene names: AAS101, AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P1
Unit cell:
a: 43.199Å b: 63.723Å c: 73.258Å
α: 66.64° β: 89.99° γ: 90.3°
R-values:
R R work R free
0.249 0.249 0.304
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Gestalt-binding of tropomyosin to actin filaments.
Holmes et al. (2008)
6 more

3 mentions without citation

Two-crystal structures of tropomyosin C-terminal fragment 176-273: exposure of the hydrophobic core to the solvent destabilizes the tropomyosin molecule.
Minakata et al. (2008)
2 more