› 2efy

X-ray diffraction
2.35Å resolution

Crystal Structure of T.th. HB8 O-acetylserine sulfhydrylase Complexed with 4-Acetylbutyric acid

Released:
DOI: 10.2210/pdb2efy/pdb
PDB entry 2efy coloured by chain, front view.
PDB entry 2efy coloured by chain, side view.
PDB entry 2efy coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry author: Goto M

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 33.07 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLE6 (Residues: 1-304; Coverage: 100%)
Gene name: TTHA0347
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand 4AT 2 x 4AT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P43
Unit cell:
a: 100.76Å b: 100.76Å c: 65.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.238
Expression system: Escherichia coli

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