› 2egg

X-ray diffraction
2.25Å resolution

Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Geobacillus kaustophilus

Released:
DOI: 10.2210/pdb2egg/pdb
PDB entry 2egg coloured by chain, front view.
PDB entry 2egg coloured by chain, side view.
PDB entry 2egg coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Entry authors: Bagautdinov B, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-177370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 32.88 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KWX7 (Residues: 1-276; Coverage: 100%)
Gene names: GK2524, aroE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I23
Unit cell:
a: 171.394Å b: 171.394Å c: 171.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.255
Expression system: Escherichia coli BL21(DE3)

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