› 2egu

X-ray diffraction
1.9Å resolution

Crystal structure of O-acetylserine sulfhydrase from Geobacillus kaustophilus HTA426

Released:
DOI: 10.2210/pdb2egu/pdb
PDB entry 2egu coloured by chain, front view.
PDB entry 2egu coloured by chain, side view.
PDB entry 2egu coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Entry authors: Rehse PH, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 32.56 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L3S8 (Residues: 1-308; Coverage: 100%)
Gene name: GK0065
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P43212
Unit cell:
a: 72.25Å b: 72.25Å c: 127.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.219
Expression system: Escherichia coli

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