› 2egw

X-ray diffraction
2.8Å resolution

Crystal structure of rRNA methyltransferase with SAH ligand

Released:
DOI: 10.2210/pdb2egw/pdb
PDB entry 2egw coloured by chain, front view.
PDB entry 2egw coloured by chain, side view.
PDB entry 2egw coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Dong X, Shirouzu M, Bessho Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.73 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66552 (Residues: 1-229; Coverage: 100%)
Gene names: aq_165, rsmE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 112.637Å b: 38.774Å c: 115.286Å
α: 90° β: 115.73° γ: 90°
R-values:
R R work R free
0.226 0.226 0.307
Expression system: Escherichia coli

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