› 2ehj

X-ray diffraction
2.8Å resolution

Structure of Uracil phosphoribosyl transferase

Released:
DOI: 10.2210/pdb2ehj/pdb
PDB entry 2ehj coloured by chain, front view.
PDB entry 2ehj coloured by chain, side view.
PDB entry 2ehj coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Lokanath NK, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 22.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8F0 (Residues: 2-208; Coverage: 100%)
Gene names: JW2483, b2498, upp, uraP
Sequence domains: Uracil phosphoribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 19 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 91.312Å b: 91.312Å c: 266.911Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.229
Expression system: Escherichia coli

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