› 2eii

X-ray diffraction
1.88Å resolution

Crystal analysis of delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus with bound L-valine and NAD.

Released:
DOI: 10.2210/pdb2eii/pdb
PDB entry 2eii coloured by chain, front view.
PDB entry 2eii coloured by chain, side view.
PDB entry 2eii coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Inagaki E, Sakamoto K, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-177922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamate gamma-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 516 amino acids
Theoretical weight: 57.11 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SI02 (Residues: 1-516; Coverage: 100%)
Gene name: TTHA1578
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
4 bound ligands:
Ligand ACT 4 x ACT
Ligand NA 2 x NA
Ligand VAL 2 x VAL
Ligand MPD 14 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: R3
Unit cell:
a: 102.208Å b: 102.208Å c: 278.432Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.136 0.134 0.168
Expression system: Escherichia coli BL21(DE3)

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