› 2ej0

X-ray diffraction
1.6Å resolution

Crystal Structure of T.th.HB8 Branched-Chain Amino Acid Aminotransferase with Pyridoxamine 5'-phosphate

Released:
DOI: 10.2210/pdb2ej0/pdb
PDB entry 2ej0 coloured by chain, front view.
PDB entry 2ej0 coloured by chain, side view.
PDB entry 2ej0 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry author: Goto M

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 308 amino acids
Theoretical weight: 34.07 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SM19 (Residues: 1-308; Coverage: 100%)
Gene names: TTHA0124, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 6 x PMP
1 bound ligand:
Ligand MPD 8 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 115.88Å b: 143.69Å c: 146.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.194
Expression system: Escherichia coli BL21(DE3)

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