› 2ej9

X-ray diffraction
2Å resolution

Crystal Structure Of Biotin Protein Ligase From Methanococcus jannaschii

Released:
DOI: 10.2210/pdb2ej9/pdb
PDB entry 2ej9 coloured by chain, front view.
PDB entry 2ej9 coloured by chain, side view.
PDB entry 2ej9 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Bagautdinov B, Bagautdinova S, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative biotin ligase Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 27.22 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59014 (Residues: 1-237; Coverage: 100%)
Gene name: MJ1619
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand BTN 1 x BTN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 117.114Å b: 61.843Å c: 42.438Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.216 0.216 0.244
Expression system: Escherichia coli

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