› 2ejv

X-ray diffraction
2.55Å resolution

Crystal structure of threonine 3-dehydrogenase complexed with NAD+

Released:
DOI: 10.2210/pdb2ejv/pdb
PDB entry 2ejv coloured by chain, front view.
PDB entry 2ejv coloured by chain, side view.
PDB entry 2ejv coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Omi R, Yao T, Goto M, Miyahara I, Hirotsu K

Function and Biology Details

Reaction catalysed: 
L-threonine + NAD(+) = L-2-amino-3-oxobutanoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-threonine 3-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 36.68 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKS4 (Residues: 1-343; Coverage: 100%)
Gene names: TTHA0569, tdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P6122
Unit cell:
a: 135.758Å b: 135.758Å c: 268.593Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.236
Expression system: Escherichia coli

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