› 2enw

X-ray diffraction
2.1Å resolution

Mutant Y92H structure of TTHB049 from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2enw/pdb
PDB entry 2enw coloured by chain, front view.
PDB entry 2enw coloured by chain, side view.
PDB entry 2enw coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Shimizu K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Adenosylcobalamin 5'-phosphate + H(2)O = coenzyme B12 + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ribazole-5'-phosphate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.6 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WB3 (Residues: 1-177; Coverage: 100%)
Gene name: TTHB049
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 84.974Å b: 91.988Å c: 110.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.213
Expression system: Escherichia coli BL21(DE3)

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