› 2ep7

X-ray diffraction
2.3Å resolution

Structural study of Project ID aq_1065 from Aquifex aeolicus VF5

Released:
DOI: 10.2210/pdb2ep7/pdb
PDB entry 2ep7 coloured by chain, front view.
PDB entry 2ep7 coloured by chain, side view.
PDB entry 2ep7 coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-130457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 37.66 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67161 (Residues: 1-342; Coverage: 100%)
Gene names: aq_1065, gap
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I212121
Unit cell:
a: 109.19Å b: 115.437Å c: 131.484Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.215
Expression system: Escherichia coli

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