› 2eq8

X-ray diffraction
1.94Å resolution

Crystal structure of lipoamide dehydrogenase from thermus thermophilus HB8 with psbdp

Released:
DOI: 10.2210/pdb2eq8/pdb
PDB entry 2eq8 coloured by chain, front view.
PDB entry 2eq8 coloured by chain, side view.
PDB entry 2eq8 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Nakai T, Kamiya N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178207 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydrolipoyl dehydrogenase Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 464 amino acids
Theoretical weight: 49.15 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLR0 (Residues: 1-464; Coverage: 100%)
Gene name: TTHA0233
Sequence domains:
Structure domains:
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex Chains: C, F
Molecule details ›
Chains: C, F
Length: 40 amino acids
Theoretical weight: 4.29 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided
UniProt:
  • Canonical: Q5SLV9 (Residues: 125-164; Coverage: 10%)
Gene name: TTHA0184
Sequence domains: e3 binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 85.758Å b: 104.082Å c: 112.856Å
α: 90° β: 107.3° γ: 90°
R-values:
R R work R free
0.207 0.207 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links