› 2eqa

X-ray diffraction
1.8Å resolution

Crystal Structure of the hypothetical Sua5 protein from Sulfolobus tokodaii

Released:
DOI: 10.2210/pdb2eqa/pdb
PDB entry 2eqa coloured by chain, front view.
PDB entry 2eqa coloured by chain, side view.
PDB entry 2eqa coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii
Primary publication:
X-ray crystal structure of a hypothetical Sua5 protein from Sulfolobus tokodaii strain 7.
Agari Y, Sato S, Wakamatsu T, Bessho Y, Ebihara A, Yokoyama S, Kuramitsu S, Shinkai A
Proteins 70 1108-11 (2008)
PMID: 18004774

Function and Biology Details

Reaction catalysed: 
L-threonine + ATP + HCO(3)(-) = L-threonylcarbamoyladenylate + diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonylcarbamoyl-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 39.08 KDa
Source organism: Sulfurisphaera tokodaii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q970S6 (Residues: 1-352; Coverage: 100%)
Gene names: STK_15260, sua5
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand AMP 1 x AMP
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C222
Unit cell:
a: 85.391Å b: 120.843Å c: 67.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.233
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The O-carbamoyltransferase TobZ catalyzes an ancient enzymatic reaction.
Parthier et al. (2012)
4 more

5 mentions without citation

A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
El Yacoubi et al. (2011)
4 more