2er8

X-ray diffraction
2.85Å resolution

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-115209 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein LEU3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 72 amino acids
Theoretical weight: 8.6 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P08638 (Residues: 32-103; Coverage: 8%)
Gene names: L9324.1, LEU3, YLR451W
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains: Zn(2)-C6 fungal-type DNA-binding domain
5'-D(*CP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*G)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6422
Unit cell:
a: 107.5Å b: 107.5Å c: 218.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.265
Expression systems:
  • Escherichia coli
  • Not provided