› 2erc

X-ray diffraction
3.03Å resolution

CRYSTAL STRUCTURE OF ERMC' A RRNA-METHYL TRANSFERASE

Released:
DOI: 10.2210/pdb2erc/pdb
PDB entry 2erc coloured by chain, front view.
PDB entry 2erc coloured by chain, side view.
PDB entry 2erc coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria.
Bussiere DE, Muchmore SW, Dealwis CG, Schluckebier G, Nienaber VL, Edalji RP, Walter KA, Ladror US, Holzman TF, Abad-Zapatero C
Biochemistry 37 7103-12 (1998)
PMID: 9585521

Function and Biology Details

Reaction catalysed: 
2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA adenine N-6-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 28.96 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P13956 (Residues: 1-244; Coverage: 100%)
Gene name: ermC'
Sequence domains: Ribosomal RNA adenine dimethylase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6
Unit cell:
a: 146.9Å b: 146.9Å c: 57.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.313
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular mechanisms that confer antibacterial drug resistance.
Walsh C. (2000)
2 more

5 mentions without citation

AdoMet-dependent methylation, DNA methyltransferases and base flipping.
Cheng et al. (2001)
4 more