› 2erg

X-ray diffraction
3.15Å resolution

Crystal Structure of Leu3 DNA-binding domain with a single H50C mutation complexed with a 15mer DNA duplex

Released:
DOI: 10.2210/pdb2erg/pdb
PDB entry 2erg coloured by chain, front view.
PDB entry 2erg coloured by chain, side view.
PDB entry 2erg coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein.
Fitzgerald MX, Rojas JR, Kim JM, Kohlhaw GB, Marmorstein R
Structure 14 725-35 (2006)
PMID: 16615914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115211 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein LEU3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P08638 (Residues: 32-103; Coverage: 8%)
Gene names: L9324.1, LEU3, YLR451W
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains: Zn(2)-C6 fungal-type DNA-binding domain
5'-D(*TP*TP*GP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*CP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P62
Unit cell:
a: 66.768Å b: 66.768Å c: 122.745Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.273 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

A fungal family of transcriptional regulators: the zinc cluster proteins.
MacPherson et al. (2006)
12 other articles