› 2erk

X-ray diffraction
2.4Å resolution

PHOSPHORYLATED MAP KINASE ERK2

Released:
DOI: 10.2210/pdb2erk/pdb
PDB entry 2erk coloured by chain, front view.
PDB entry 2erk coloured by chain, side view.
PDB entry 2erk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Activation mechanism of the MAP kinase ERK2 by dual phosphorylation.
Canagarajah BJ, Khokhlatchev A, Cobb MH, Goldsmith EJ
Cell 90 859-69 (1997)
PMID: 9298898

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 42.39 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63086 (Residues: 1-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 1 x TPO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P41212
Unit cell:
a: 92.5Å b: 92.5Å c: 103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

81 review citations

Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation.
Kyriakis et al. (2001)
80 more

70 mentions without citation

Post-translational modification: nature's escape from genetic imprisonment and the basis for dynamic information encoding.
Prabakaran et al. (2012)
69 more