› 2etj

X-ray diffraction
1.74Å resolution

Crystal structure of Ribonuclease HII (EC 3.1.26.4) (RNase HII) (tm0915) from THERMOTOGA MARITIMA at 1.74 A resolution

Released:
DOI: 10.2210/pdb2etj/pdb
PDB entry 2etj coloured by chain, front view.
PDB entry 2etj coloured by chain, side view.
PDB entry 2etj coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.41 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X017 (Residues: 1-238; Coverage: 100%)
Gene names: TM_0915, rnhB
Sequence domains: Ribonuclease HII
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand EDO 5 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P31
Unit cell:
a: 51.821Å b: 51.821Å c: 76.293Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.219
Expression system: Escherichia coli

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