› 2etw

X-ray diffraction
1.67Å resolution

Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes

Released:
DOI: 10.2210/pdb2etw/pdb
PDB entry 2etw coloured by chain, front view.
PDB entry 2etw coloured by chain, side view.
PDB entry 2etw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes.
Lamoureux JS, Glover JN
Structure 14 555-65 (2006)
PMID: 16531239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115214 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Meiosis-specific transcription factor NDT80 Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 39.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38830 (Residues: 1-340; Coverage: 54%)
Gene names: DAS1, NDT80, YHR124W
Sequence domains: NDT80 / PhoG like DNA-binding family
Structure domains: NDT80 DNA-binding domain
5'-D(*TP*GP*CP*CP*AP*CP*AP*CP*AP*AP*AP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*AP*GP*TP*TP*TP*TP*TP*GP*TP*GP*TP*GP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 70.299Å b: 78.919Å c: 161.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.199
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

A common mode of recognition for methylated CpG.
Liu et al. (2013)
1 more

2 mentions without citation

Flexibility-rigidity index for protein-nucleic acid flexibility and fluctuation analysis.
Opron et al. (2016)
1 more