› 2eve

X-ray diffraction
1.6Å resolution

X-Ray Crystal Structure of Protein PSPTO5229 from Pseudomonas syringae. Northeast Structural Genomics Consortium Target PsR62

Released:
DOI: 10.2210/pdb2eve/pdb
PDB entry 2eve coloured by chain, front view.
PDB entry 2eve coloured by chain, side view.
PDB entry 2eve coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Structural genomics reveals EVE as a new ASCH/PUA-related domain.
Bertonati C, Punta M, Fischer M, Yachdav G, Forouhar F, Zhou W, Kuzin AP, Seetharaman J, Abashidze M, Ramelot TA, Kennedy MA, Cort JR, Belachew A, Hunt JF, Tong L, Montelione GT, Rost B
Proteins 75 760-73 (2009)
PMID: 19191354

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EVE domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.1 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87UR7 (Residues: 1-149; Coverage: 100%)
Gene name: PSPTO_5229
Sequence domains: EVE domain
Structure domains: ph1033 like domains

Ligands and Environments

3 bound ligands:
Ligand MPO 1 x MPO
Ligand EDO 1 x EDO
Ligand 144 1 x 144
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 77.233Å b: 42.273Å c: 50.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Adenine methylation in eukaryotes: Apprehending the complex evolutionary history and functional potential of an epigenetic modification.
Iyer et al. (2016)
3 more

11 mentions without citation

The rough guide to in silico function prediction, or how to use sequence and structure information to predict protein function.
Punta et al. (2008)
10 more