› 2ew2

X-ray diffraction
2Å resolution

Crystal Structure of the Putative 2-Dehydropantoate 2-Reductase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb2ew2/pdb
PDB entry 2ew2 coloured by chain, front view.
PDB entry 2ew2 coloured by chain, side view.
PDB entry 2ew2 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Kim Y, Zhou M, Moy S, Clancy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182774 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydropantoate 2-reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 35.12 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q831Q5 (Residues: 1-313; Coverage: 100%)
Gene name: EF_2445
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 89.165Å b: 89.16Å c: 104.754Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.214
Expression system: Escherichia coli BL21(DE3)

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