› 2ewb

X-ray diffraction
1.85Å resolution

The crystal structure of Bovine Lens Leucine Aminopeptidase in complex with zofenoprilat

Released:
DOI: 10.2210/pdb2ewb/pdb
PDB entry 2ewb coloured by chain, front view.
PDB entry 2ewb coloured by chain, side view.
PDB entry 2ewb coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Metal ion substitution in the catalytic site greatly affects the binding of sulfhydryl-containing compounds to leucyl aminopeptidase.
Cappiello M, Alterio V, Amodeo P, Del Corso A, Scaloni A, Pedone C, Moschini R, De Donatis GM, De Simone G, Mura U
Biochemistry 45 3226-34 (2006)
PMID: 16519517

Function and Biology Details

Reactions catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of N-terminal proline from a peptide.
An (L-cysteinylglycine)-S-conjugate + H(2)O = an L-cysteine-S-conjugate + glycine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosol aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 52.87 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00727 (Residues: 33-518; Coverage: 94%)
Gene name: LAP3
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand NA 1 x NA
Ligand CO3 1 x CO3
Ligand ZED 1 x ZED
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6322
Unit cell:
a: 128.94Å b: 128.94Å c: 119.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.156 0.177

Quick links

Citations

4 review citations

Leucine aminopeptidases: diversity in structure and function.
Matsui et al. (2006)
3 more

3 mentions without citation

Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families.
Pascual Alonso et al. (2023)
2 more