› 2ewj

X-ray diffraction
2.7Å resolution

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form

Released:
DOI: 10.2210/pdb2ewj/pdb
PDB entry 2ewj coloured by chain, front view.
PDB entry 2ewj coloured by chain, side view.
PDB entry 2ewj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A molecular mousetrap determines polarity of termination of DNA replication in E. coli.
Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, Dixon NE
Cell 125 1309-19 (2006)
PMID: 16814717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115224 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication terminus site-binding protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P16525 (Residues: 1-309; Coverage: 100%)
Gene names: JW1602, b1610, tau, tus
Sequence domains: DNA replication terminus site-binding protein (Ter protein)
Structure domains:
5'-D(*T*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*CP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*TP*G*AP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IOD 3 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 62.679Å b: 62.679Å c: 251.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.303
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

Replication fork stalling at natural impediments.
Mirkin et al. (2007)
17 more

4 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
3 more