› 2ewn

X-ray diffraction
2.8Å resolution

Ecoli Biotin Repressor with co-repressor analog

Released:
DOI: 10.2210/pdb2ewn/pdb
PDB entry 2ewn coloured by chain, front view.
PDB entry 2ewn coloured by chain, side view.
PDB entry 2ewn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Co-repressor induced order and biotin repressor dimerization: a case for divergent followed by convergent evolution.
Wood ZA, Weaver LH, Brown PH, Beckett D, Matthews BW
J Mol Biol 357 509-23 (2006)
PMID: 16438984

Function and Biology Details

Reaction catalysed: 
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional ligase/repressor BirA Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06709 (Residues: 1-321; Coverage: 100%)
Gene names: JW3941, b3973, bioR, birA, dhbB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BTX 2 x BTX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 152.188Å b: 152.188Å c: 93.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.264
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Allostery: absence of a change in shape does not imply that allostery is not at play.
Tsai et al. (2008)
5 more

27 mentions without citation

Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches.
Samavarchi-Tehrani et al. (2020)
26 more