› 2exo

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE BETA-1,4-GLYCANASE CEX FROM CELLULOMONAS FIMI

Released:
DOI: 10.2210/pdb2exo/pdb
PDB entry 2exo coloured by chain, front view.
PDB entry 2exo coloured by chain, side view.
PDB entry 2exo coloured by chain, top view.
Source organism: Cellulomonas fimi
Primary publication:
Crystal structure of the catalytic domain of the beta-1,4-glycanase cex from Cellulomonas fimi.
White A, Withers SG, Gilkes NR, Rose DR
Biochemistry 33 12546-52 (1994)
PMID: 7918478

Function and Biology Details

Reactions catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase/xylanase Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.05 KDa
Source organism: Cellulomonas fimi
Expression system: Not provided
UniProt:
  • Canonical: P07986 (Residues: 42-353; Coverage: 70%)
Gene names: cex, xynB
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 88.147Å b: 88.147Å c: 81.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.266
Expression system: Not provided

Quick links

Citations

8 review citations

Xylanases, xylanase families and extremophilic xylanases.
Collins et al. (2005)
7 more

9 mentions without citation

Incorporating dipolar solvents with variable density in Poisson-Boltzmann electrostatics.
Azuara et al. (2008)
8 more