2exw

X-ray diffraction
3.2Å resolution

Crystal structure of a EcClC-Fab complex in the absence of bound ions

Released:
Model geometry
Fit model/data
Source organisms:
Primary publication:
Ion-binding properties of the ClC chloride selectivity filter.
EMBO J 25 24-33 (2006)
PMID: 16341087

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211172 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab Fragment (Heavy Chain) Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab Fragment (Light Chain) Chains: D, F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 219.834Å b: 122.436Å c: 151.344Å
α: 90° β: 128.16° γ: 90°
R-values:
R R work R free
0.268 0.268 0.314
Expression system: Escherichia coli BL21(DE3)