› 2f02

X-ray diffraction
1.9Å resolution

Crystal Structure of LacC from Enterococcus Faecalis in complex with ATP

Released:
DOI: 10.2210/pdb2f02/pdb
PDB entry 2f02 coloured by chain, front view.
PDB entry 2f02 coloured by chain, side view.
PDB entry 2f02 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Gorman J, Shapiro L, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tagatose-6-phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 35.4 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q833W9 (Residues: 2-313; Coverage: 100%)
Gene names: EF_1806, lacC
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 65.463Å b: 62.739Å c: 93.454Å
α: 90° β: 99.91° γ: 90°
R-values:
R R work R free
0.203 0.201 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links