› 2f1k

X-ray diffraction
1.55Å resolution

Crystal structure of Synechocystis arogenate dehydrogenase

Released:
DOI: 10.2210/pdb2f1k/pdb
PDB entry 2f1k coloured by chain, front view.
PDB entry 2f1k coloured by chain, side view.
PDB entry 2f1k coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Biochemical characterization and crystal structure of Synechocystis arogenate dehydrogenase provide insights into catalytic reaction.
Legrand P, Dumas R, Seux M, Rippert P, Ravelli R, Ferrer JL, Matringe M
Structure 14 767-76 (2006)
PMID: 16615917

Function and Biology Details

Reaction catalysed: 
L-arogenate + NAD(+) = L-tyrosine + NADH + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prephenate/arogenate dehydrogenase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 279 amino acids
Theoretical weight: 30.32 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73906 (Residues: 1-279; Coverage: 100%)
Gene name: tyrA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand TRS 2 x TRS
2 modified residues:
Ligand OMT 4 x OMT
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, null
Spacegroup: P21
Unit cell:
a: 66.15Å b: 70.85Å c: 104.49Å
α: 90° β: 90.3° γ: 90°
R-values:
R R work R free
0.19 0.189 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
2 more

7 mentions without citation

The crystal structure of Aquifex aeolicus prephenate dehydrogenase reveals the mode of tyrosine inhibition.
Sun et al. (2009)
6 more