› 2f1m

X-ray diffraction
2.71Å resolution

Conformational flexibility in the multidrug efflux system protein AcrA

Released:
DOI: 10.2210/pdb2f1m/pdb
PDB entry 2f1m coloured by chain, front view.
PDB entry 2f1m coloured by chain, side view.
PDB entry 2f1m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conformational flexibility in the multidrug efflux system protein AcrA.
Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P
Structure 14 577-87 (2006)
PMID: 16531241

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 277 amino acids
Theoretical weight: 30.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE06 (Residues: 45-312; Coverage: 72%)
Gene names: JW0452, acrA, b0463, lir, mtcA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 88.788Å b: 100.031Å c: 332.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.275
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Multidrug resistance in bacteria.
Nikaido H. (2009)
37 more

35 mentions without citation

Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea.
Unemo et al. (2012)
34 more