› 2f3i

Solution NMR

Solution Structure of a Subunit of RNA Polymerase II

Released:
DOI: 10.2210/pdb2f3i/pdb
PDB entry 2f3i coloured by chain, ensemble of 21 models, front view.
PDB entry 2f3i coloured by chain, ensemble of 21 models, side view.
PDB entry 2f3i coloured by chain, ensemble of 21 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural, biochemical, and dynamic characterizations of the hRPB8 subunit of human RNA polymerases.
Kang X, Hu Y, Li Y, Guo X, Jiang X, Lai L, Xia B, Jin C
J Biol Chem 281 18216-26 (2006)
PMID: 16632472

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52434 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Evolution of multisubunit RNA polymerases in the three domains of life.
Werner et al. (2011)
2 more

3 mentions without citation

Integrative structure modeling of macromolecular assemblies from proteomics data.
Lasker et al. (2010)
2 more