› 2f3t

X-ray diffraction
3.16Å resolution

Crystal Structure Of E.coli Guanylate Kinase In Complex With Ganciclovir monophosphate

Released:
DOI: 10.2210/pdb2f3t/pdb
PDB entry 2f3t coloured by chain, front view.
PDB entry 2f3t coloured by chain, side view.
PDB entry 2f3t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G.
Hible G, Daalova P, Gilles AM, Cherfils J
Biochimie 88 1157-64 (2006)
PMID: 16690197

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 207 amino acids
Theoretical weight: 23.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60546 (Residues: 1-207; Coverage: 100%)
Gene names: JW3623, b3648, gmk, spoR
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LGP 4 x LGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 107.753Å b: 107.753Å c: 272.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.29
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

FBA reveals guanylate kinase as a potential target for antiviral therapies against SARS-CoV-2.
Renz et al. (2020)
10 more