› 2f4t

X-ray diffraction
3Å resolution

Asite RNA + designer antibiotic

Released:
DOI: 10.2210/pdb2f4t/pdb
PDB entry 2f4t coloured by chain, front view.
PDB entry 2f4t coloured by chain, side view.
PDB entry 2f4t coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Interactions of designer antibiotics and the bacterial ribosomal aminoacyl-tRNA site.
Murray JB, Meroueh SO, Russell RJ, Lentzen G, Haddad J, Mobashery S
Chem Biol 13 129-38 (2006)
PMID: 16492561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196345 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.74 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*UP*UP*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand AB9 1 x AB9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 45.976Å b: 32.926Å c: 46.414Å
α: 90° β: 95.62° γ: 90°
R-values:
R R work R free
0.211 0.211 0.267
Expression system: Not provided

Quick links

Citations

5 review citations

Molecular dynamics simulations of RNA: an in silico single molecule approach.
McDowell et al. (2007)
4 more

2 mentions without citation

Novel insights of structure-based modeling for RNA-targeted drug discovery.
Chen et al. (2012)
1 more