› 2f58

X-ray diffraction
2.8Å resolution

IGG1 FAB FRAGMENT (58.2) COMPLEX WITH 12-RESIDUE CYCLIC PEPTIDE (INCLUDING RESIDUES 315-324 OF HIV-1 GP120) (MN ISOLATE)

Released:
DOI: 10.2210/pdb2f58/pdb
PDB entry 2f58 coloured by chain, front view.
PDB entry 2f58 coloured by chain, side view.
PDB entry 2f58 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Dual conformations for the HIV-1 gp120 V3 loop in complexes with different neutralizing fabs.
Stanfield R, Cabezas E, Satterthwait A, Stura E, Profy A, Wilson I
Structure 7 131-42 (1999)
PMID: 10368281

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208467 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig kappa chain V-III region PC 7183 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01666 (Residues: 1-111; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
PROTEIN (IGG1 FAB 58.2 ANTIBODY (HEAVY CHAIN)) Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.86 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
PROTEIN (HIV-1 GP120) Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.03 KDa

Ligands and Environments

1 bound ligand:
Ligand ARN 1 x ARN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 96.08Å b: 114.72Å c: 49.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.305

Quick links

Citations

7 review citations

V3: HIV's switch-hitter.
Hartley et al. (2005)
6 more

5 mentions without citation

Prediction of transmembrane helix orientation in polytopic membrane proteins.
Adamian et al. (2006)
4 more