› 2f5m

X-ray diffraction
1.95Å resolution

Cross-linked barnase soaked in bromo-ethanol

Released:
DOI: 10.2210/pdb2f5m/pdb
PDB entry 2f5m coloured by chain, front view.
PDB entry 2f5m coloured by chain, side view.
PDB entry 2f5m coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations.
Salem M, Mauguen Y, Prangé T
Biochim Biophys Acta 1764 903-12 (2006)
PMID: 16600702

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 108 amino acids
Theoretical weight: 12.2 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 50-157; Coverage: 88%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand BRJ 2 x BRJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P32
Unit cell:
a: 59.28Å b: 59.28Å c: 81.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Counteraction of urea by trimethylamine N-oxide is due to direct interaction.
Meersman et al. (2009)
5 more