› 2f6k

X-ray diffraction
2.5Å resolution

Crystal Structure of Amidohydrorolase II; Northeast Structural Genomics Target LpR24

Released:
DOI: 10.2210/pdb2f6k/pdb
PDB entry 2f6k coloured by chain, front view.
PDB entry 2f6k coloured by chain, side view.
PDB entry 2f6k coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum WCFS1
Entry authors: Das K, Xiao R, Acton T, Ma L, Arnold E, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
6-methylsalicylate = 3-methylphenol + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-methylsalicylate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.85 KDa
Source organism: Lactiplantibacillus plantarum WCFS1
Expression system: Escherichia coli
UniProt:
  • Canonical: F9US96 (Residues: 1-307; Coverage: 100%)
Gene name: lp_2961
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4322
Unit cell:
a: 82.62Å b: 82.62Å c: 240.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.94 0.192 0.225
Expression system: Escherichia coli

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