› 2f7a

X-ray diffraction
1.9Å resolution

BenM effector binding domain with its effector, cis,cis-muconate

Released:
DOI: 10.2210/pdb2f7a/pdb
PDB entry 2f7a coloured by chain, front view.
PDB entry 2f7a coloured by chain, side view.
PDB entry 2f7a coloured by chain, top view.
Source organism: Acinetobacter baylyi
Primary publication:
Distinct effector-binding sites enable synergistic transcriptional activation by BenM, a LysR-type regulator.
Ezezika OC, Haddad S, Clark TJ, Neidle EL, Momany C
J Mol Biol 367 616-29 (2007)
PMID: 17291527

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator BenM Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.4 KDa
Source organism: Acinetobacter baylyi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68014 (Residues: 81-304; Coverage: 74%)
Gene names: ACIAD1435, benM, benR
Sequence domains: LysR substrate binding domain
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand BEZ 2 x BEZ
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
Ligand CCU 1 x CCU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 64.741Å b: 65.777Å c: 117.974Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins.
Maddocks et al. (2008)
4 more

3 mentions without citation

Oligomerization of BenM, a LysR-type transcriptional regulator: structural basis for the aggregation of proteins in this family.
Ezezika et al. (2007)
2 more